Beilstein J. Nanotechnol.2022,13, 1458–1472, doi:10.3762/bjnano.13.120
Southeast Asian boxturtle, Cuora amboinensis, is an ecologically important endangered species which needs an onsite monitoring device to protect it from extinction. An electrochemical DNA biosensor was developed to detect the C. amboinensis mitochondrial cytochrome b gene based on an in silico designed
in the range of 1 × 10−11–5 × 10−6 M with a limit of detection of 0.85 × 10−12 M. This indicates that the proposed biosensor has the potential to be applied for the detection of real turtle species.
Keywords: boxturtle; DNA detection; electrochemical DNA biosensor; nanocomposite; screen-printed
carbon electrode; Introduction
The Southeast Asian boxturtle (BT), Cuora amboinensis, is an endangered and protected turtle species. Due to its high value as an exotic food item and in traditional medicine, it ends up being a profitable item in the illicit wildlife trade [1]. Turtles contain active
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Figure 1:
The Cuora amboinensis specific probe (31 bases) and corresponding aligned sequences. A, T, G, C (A,...